Invitrogen™ E2F1 Monoclonal Antibody (16G7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA531589

Catalog No. PIMA531589

Description

Keep as concentrated solution. Positive Control: Jurkat.

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Specifications

• Antigen: E2F1
• Classification: Monoclonal
• Concentration: 1.86 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q01094, Q61501
• Gene Symbols: E2F1
• Immunogen: The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 16G7
• Conjugate: Unconjugated
• Gene: E2F1
• Gene Alias: E2F transcription factor 1; E2F1; E2F-1; mKIAA4009; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; retinoblastoma-binding protein 3; Transcription factor E2F1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 13555, 1869
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid