Invitrogen™ E2F1 Monoclonal Antibody (8G9)

Catalog No.	Quantity
PIMA517070	100 μg

Catalog No. PIMA517070 Supplier Invitrogen™ Supplier No. MA517070

Description

Mouse Monoclonal Antibody

MA5-17070 targets E2F1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples. The MA5-17070 immunogen is purified recombinant fragment of human E2F1 (amino acids: 69-223) expressed in E. Coli. MA5-17070 detects E2F1 which has a predicted molecular weight of approximately 46.9kDa.

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Specifications

• Antigen: E2F1
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 8G9
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: E2F1
• Gene Accession No.: Q01094, Q61501
• Gene Alias: E2F transcription factor 1; E2F1; E2F-1; mKIAA4009; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; retinoblastoma-binding protein 3; Transcription factor E2F1
• Gene Symbols: E2F1
• Host Species: Mouse
• Immunogen: Purified recombinant fragment of human E2F1 (amino acids: 69-223) expressed in E. Coli.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13555, 1869
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1