Invitrogen™ E2F6 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595059

Catalog No. PIPA595059

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: HELA whole cell, COLO320 whole cell, A549 whole cell, MCF-7 whole cell, SMMC whole cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.

Specifications

• Antigen: E2F6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: E2F6
• Gene Alias: AI462434; E2F transcription factor 6; E2f6; E2F-6; E2F6b; E2F-binding site-modulating activity protein; EMA; MGC111545; Transcription factor E2F6; transcriptional repressor E2F6
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1876, 313978, 50496
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: O54917, O75461
• Gene Symbols: E2F6
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E2F6 (162-177aa KDCAQQLFELTDDKEN).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG