Invitrogen™ E4BP4 (NFIL3) Monoclonal Antibody (MABA223), PE, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
12-981-242	Invitrogen™ 12981242	100 Tests

Description

Description: This MABA223 monoclonal antibody recognizes human E4BP4 (NFIL3), a basic leucine zipper transcription factor first identified for its binding to the promoters of the adenovirus E4 protein and human IL-3. E4BP4 is involved in the transcriptional control of the mammalian circadian clock, motor neuron growth and survival, regulation of von Willbrand factor expression, and osteoblast function. In addition, it is required for the development of NK and NKT cells as well as CD8a+ dendritic cells. Although relatively high levels of mRNA are reported in resting NK and NKT cells, studies at eBioscience have observed expression of E4BP4 protein in lymphocytes only after activation. Applications Reported: This MABA223 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MABA223 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).

Specifications

• Antigen: E4BP4 (NFIL3)
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q16649
• Gene ID (Entrez): 4783
• Immunogen: 21H2-hNFIL3 (aa 163-462).
• Product Type: Antibody

• Clone: MABA223
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: NFIL3
• Gene Alias: AV225605; E4 promoter binding-protein 4; E4 promoter-binding protein 4; E4BP4; Embryo implantation-related NFIL3/E4BP4-like transcription factor; IL3BP1; Interleukin-3 promoter transcriptional activator; interleukin-3-binding protein 1; NF IL3A; NFIL3; NFIL3A; NF-IL3A; nuclear factor interleukin 3 regulated protein; nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; nuclear factor, interleukin 3, regulated; OTTHUMP00000021633; transcriptional activator NF-IL3A
• Gene Symbols: NFIL3
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography