Invitrogen™ EAAC1 Monoclonal Antibody (N180/41), PerCP

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545549

Catalog No. PIMA545549

Description

5 μg of MA5-45549 was sufficient for immunoprecipitation of EAAC1 from 50 μg of rat brain lysate. Detects approximately 60kDa. This antibody was formerly sold as clone S180-41.

L-glutamate is the major excitatory neurotransmitter in mammalian central nervous systems. To maintain glutamate levels below excitoxic levels, excess glutamate at excitatory synaptic clefts is removed by ion-coupled glutamate transporters. Four glutamate transporters have been identified: the sodium-dependent GLAST-1, GLT-1, EAAC-1 and the chloride-dependent EAAT-4. EAAC-1 is expressed in the CNS and also found in kidney and small intestine. Structural features of glutamate transporters are believed to include eight membrane-spanning alpha-helices and a loop-pore structure which is unique among secondary transporters but may resemble loop-pores found in ion channels. A second distinctive structural feature is the presence of a highly amphipathic membrane-spanning helix that provides a hydrophilic path through the membrane. Mice with homozygous deletions of the EAAC-1 transporter gene develop dicarboxylic aminoaciduria and display reduced spontaneous locomotor activity although no neurodegeneration was observed over a period of 12 months.

Specifications

• Antigen: EAAC1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: P43005, P51907
• Gene Symbols: Slc1a1
• Immunogen: Fusion protein amino acids 1-524 (full length) of human EAAC1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: N180/41
• Conjugate: PerCP
• Gene: Slc1a1
• Gene Alias: D130048G10Rik; DCBXA; EAAC1; EAAC2; EAAT3; excitatory amino acid carrier 1; excitatory amino acid carrier 2; excitatory amino acid transporter 3; excitatory amino acid transporter-3; excitatory amino-acid carrier 1; glutamate transporter mEAAC2; MEAAC1; Neuronal and epithelial glutamate transporter; REAAC1; SCZD18; SLC1A1; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1; Solute carrier family 1 A1 (brain glutamate transporter); solute carrier family 1 member 1; solute carrier family 1, member 1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 25550, 6505
• Content And Storage: 4°C
• Form: Liquid