Invitrogen™ EB1 Monoclonal Antibody (KT51)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA547663

Catalog No. PIMA547663

Description

The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells.

EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. The MAPRE genes encode the EB1 family proteins. The human MAPRE genes MAPRE1, MAPRE2, and MAPRE3 code for EB1, RP1, and EBF3 proteins, respectively. EB1 family members appear to be expressed ubiquitously. The EB1 (MAPRE1) gene, at 20q11. 2, is fused to MLL in an adult patient with pro-B acute lymphoblastic leukemia. EB1 is a microtubule-associated protein that interacts with the colorectal adenomatous polyposis coli tumor-suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability.

Specifications

• Antigen: EB1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: Q15691, Q61166, Q66HR2
• Gene Symbols: Mapre1
• Immunogen: Clone KT51 was generated from a GST fusion protein containing amino acids from the C-terminal region of mouse EB1. This sequence is highly conserved in human and rat EB1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot, Immunocytochemistry
• Clone: KT51
• Conjugate: Unconjugated
• Gene: Mapre1
• Gene Alias: 5530600P05Rik; adenomatosis polyposis coli binding protein Eb1; adenomatous polyposis coli-binding protein EB1; AI462499; AI504412; APC-binding protein EB1; AW260097; BIM1p; D2Ertd459e; Eb1; end-binding protein 1; Mapre1; MGC117374; MGC129946; microtubule associated protein RP/EB family member 1; Microtubule-associated protein RP/EB family member 1; microtubule-associated protein RP/EB family member 1-like protein; microtubule-associated protein, RP/EB family, member 1
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 114764, 13589, 22919
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid