Invitrogen™ ECH1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527920

Catalog No. PIPA527920

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (80%), Rat (83%), Pig (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Specifications

• Antigen: ECH1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ECH1
• Gene Alias: AA617331; delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; delta3,5-delta2,4-dienoyl-coenzyme A isomerase; dienoyl-CoA isomerase; ECH1; enoyl CoA hydratase 1, peroxisomal; enoyl coenzyme A hydratase 1, peroxisomal; enoyl hydratase-like protein peroxisomal; enoyl hydratase-like protein, peroxisomal; enoyl-CoA hydratase 1; enoyl-CoA hydratase 1, peroxisomal; HPXEL; Peroxisomal enoyl hydratase-like protein; peroxisomal enoyl-CoA hydratase 1; peroxisomal/mitochondrial dienoyl-CoA isomerase; Pxel
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1891, 51798
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O35459, Q13011
• Gene Symbols: ECH1
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ECH1. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG