MilliporeSigma™ Ecm²9 (Ab Ecm²9-3), Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABS1052MI	100 μL	Unconjugated

Catalog No. ABS1052MI Supplier MilliporeSigma™ Supplier No. ABS1052

Description

Rabbit Polyclonal Antibody

Proteasome-associated protein ECM29 homolog (UniProt Q5VYK3; also known as Ecm29, Extracellular mutant protein 29 homolog) is encoded by the ECM29 (also known as KIAA0368) gene (Gene ID 23392) in human. Ecm29 is a large adaptor protein found exclusively on the 26S enzyme to form a subset of 26S proteasomes (Ecm proteasomes), where it functions to link 26S proteasome to molecular motors, centrosome, endoplasmic reticulum, and endocytic vesicles. Ubiquitinated proteins containing K48- and K63-linked polyubiquitin chains accumulate in the centrosomes during M phase relative to S phase, shRNA-mediated Ecm29 knockdown is found to result in a decreased accumulation of ubiquitinated proteins in the centrosomes. Ecm29 downregulation is also reported to result in an elevated level of toll-like receptor 3 (TLR3) and enhanced TLR3 signaling as a result of impaired autophagy. Ecm29 is produced as a 1845-amino acid protein whose initiator methionine is removed posttranslationally to yiled the mature protein (a.a. 2-1845) composed almost entirely of Huntingtin-elongation factor 3-protein phosphatase 2A-Tor1 (HEAT) repeats, 27 in total (a.a. 34-1828).

Specifications

• Antigen: Ecm29 (Ab Ecm29-3)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene: ECM29, KIAA0368
• Gene Accession No.: Q5VYK3
• Host Species: Rabbit
• Immunogen: Ovalbumin-conjugated linear peptide corresponding to the C-terminal sequence of human Ecm29.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): XP_005251907
• Test Specificity: This polyclonal antiserum (Ab Ecm29-3) stained both early and late endosomes in HeLa cells and detected the ∽205 kDa target band in HeLa cell lysate and in various mouse tissue homogenates. A high molecular weight Ecm29 isoform was also detected by this antiserum in mouse brain homogenate (Gorbea, C., et al., (2004). J. Biol. Chem. 2279(52):54849-54861).
• Target Species: Bovine
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.