Edvotek™ Human PCR Tool Box

Description

Includes

Instructions, D1S80 Primer Mix Concentrate, PV92 Primer Mix Concentrate, Mitochondrial Primer Mix Concentrate, EdvoQuick™ DNA Ladder, Control DNA Concentrate, TE Buffer, Proteinase K, UltraSpec-Agarose, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Stain, Microcentrifuge & PCR Tubes, Disposable Plastic Cups, Salt Packets, & Wax Beads

Requires

Micropipets to measure between 5 and 50μL; Tips; Water bath; Thermal cycler; Electrophoresis apparatus; Power supply; Microwave or hotplate; UV transilluminator

Carry out three Polymerase Chain Reactions (PCR) experiments in your class at once.

Provides three sets of primers to carry out PCR amplification of:

• Alu element (PV92) on Chromosome 16
• VNTR locus (D1S80) on Chromosome 1
• Two regions of the mitochondrial gene

Experiment Times

• • Extraction 50 mins
  • PCR Setup10 mins
  • PCR 2 hours
  • Electrophoresis 60 mins
  • Staining 5 mins to overnight

Order Info

For 25 Students

Specifications

• Product Type: PCR Kit
• For Use With (Application): Provides three sets of primers to carry out the pcr amplification of alu element (pv92) on chromosome 16, the vntr locus (d1s80) on chromosome 1 and two regions of the mitochondrial gene
• Includes: Instructions; proteinase k; pcr edvobeads™; control and primer dna; microtubes; TE buffer; agarose; dna ladder; practice gel loading solution; gel loading dye; electrophoresis buffer; instastain™ ethidium bromide
• Time Range: 2 Hr.
• Content And Storage: Refrigerated
• Class Size: 25 Students