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Edvotek™ Ion-Exchange Chromatography Kit
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Catalog No. S94617 Shop All EDVOTEK Products
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S94617 10 Lab Groups
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Catalog No. S94617 Supplier Edvotek™ Supplier No. 243
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Description

Includes

Materials for six separations: ion exchanger, CM-Cellulose; concentrated Potassium Acetate buffer, pH 6.0; dye mixture: blue dye, Cytochrome c; Cytochrome c Standard (10mg/mL); chromatography columns; plastic pipets; 1mL pipet; 15mL conical tubes; microcentrifuge tubes; complete instructions; background information; and study questions

Requires

5mL test tubes; ring stand with clamps for columns; 100 or 200mL beakers or flasks; 100, 250 and 500mL graduated cylinders; distilled water; 5mL pipets and pumps; and spectrophotometer and cuvets (optional, must be able to read 1mL samples)

Learn the principles of ion-exchange chromatography.

Most biological molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically.

  • Learn the principles of ion-exchange chromatography by separating two charged molecules using a two-step salt gradient
  • Proteins are absorbed onto a charged solid support ion-exchange column and subsequently separated during elution under conditions that influence their net charge

Specifications

Grade 9 to Undergraduate
For Use With (Application) Learn The Principles of Ion-Exchange Chromatography
Includes Instructions, ion exchanger, chemical mixture, potassium acetate buffer, chromatography columns
Class Size 10 Lab Groups
Product Type Ion Exchange Chromatography Kit
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