Invitrogen™ EEF2K Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA522175

Catalog No. PIPA522175

Description

Recommended positive controls: A431, H1299, HeLa, HepG2, Raji, mouse liver, PC-12. Predicted reactivity: Mouse (91%), Rat (89%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

EEF2K is a highly conserved Serine/Threoning kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.

Specifications

• Antigen: EEF2K
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EEF2K
• Gene Alias: C86191; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; EC 2.7.11.20; eEF-2 kinase; Eef2k; eEF-2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; euk; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; kinase eEF2K; SMEF2K
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29904
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O00418
• Gene Symbols: EEF2K
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human eEF2K. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG