Invitrogen™ EGF Monoclonal Antibody (4E11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515606

Catalog No. PIMA515606

Description

MA5-15606 targets EGF in indirect ELISA, IF and WB applications and shows reactivity with Human samples. The MA5-15606 immunogen is purified recombinant fragment of human EGF expressed in E. Coli.. MA5-15606 detects EGF which has a predicted molecular weight of approximately 134kDa.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Specifications

• Antigen: EGF
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites with 0.03% sodium azide
• Gene Accession No.: P01132, P01133
• Gene Symbols: EGF
• Immunogen: Purified recombinant fragment of human EGF expressed in E. Coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 13645, 1950
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: 4E11
• Conjugate: Unconjugated
• Gene: EGF
• Gene Alias: AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1