EGF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Pepro Tech Inc 500P277BT25UG

Catalog No. 500P277BT25

Description

AA Sequence of recombinant protein: MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat EGF. Anti-Rat EGF-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Rat EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat EGF (500-P277) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat EGF. Western Blot: To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

EGF Polyclonal antibody specifically detects EGF in Rat samples. It is validated for ELISA, Western Blot

Specifications

• Antigen: EGF
• Classification: Polyclonal
• Formulation: PBS with no preservative
• Gene Accession No.: P07522
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25313
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Gene: EGF
• Gene Alias: AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
• Immunogen: E.coli-derived Recombinant Rat EGF
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Form: Lyophilized