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PeproTech EGF Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P174G1MG
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500P174G1MG 1 mg
500P174G50 50 μg
500P174G100 100 μg
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Catalog No. 500P174G1MG Supplier PeproTech Supplier No. 500P174G1MG
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Description

Goat Polyclonal Antibody

AA Sequence of recombinant protein: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR. Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine EGF. Anti-Murine EGF-specific antibody was purified by affinity chromatography employing an immobilized Murine EGF matrix. Sandwich ELISA: To detect Murine EGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine EGF (500-P174GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine EGF. Western Blot: To detect Murine EGF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
TRUSTED_SUSTAINABILITY

Specifications

Antigen EGF
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene EGF
Gene Accession No. P01132
Gene Alias AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
Gene Symbols EGF
Host Species Goat
Immunogen E.coli-derived Recombinant Murine EGF.
Purification Method Antigen affinity chromatography
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 13645
Target Species Mouse
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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