EGFR Antibody (31G7 + GFR1195), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2451510.2MG

Catalog No. NBP24515102

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

EGFR Monoclonal antibody specifically detects EGFR in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Paraffin, Flow (Cell Surface), Immunofluorescence.

Specifications

• Antigen: EGFR
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Gene Accession No.: P00533, P00533
• Gene Symbols: EGFR
• Immunogen: Human EGFR purified from A431 cells (31G7); Recombinant human EGFR protein (GFR1195)
• Quantity: 0.2 mg
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Growth and Development, Hypoxia, Signal Transduction, Tumor Suppressors, Tyrosine Kinases
• Gene ID (Entrez): 1956
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: 31G7 + GFR1195
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, Beta cellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
• Content And Storage: Store at 4C.
• Isotype: IgG

For Research Use Only