EGFR Antibody (DH8.3) - EGFRvIII, Mutant - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 5 publications

Supplier:  Novus Biologicals NBP250599

Catalog No. NBP250599

Description

EGFR Monoclonal specifically detects EGFR in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, CyTOF-ready.

Specifications

• Antigen: EGFR
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:100 - 1:2000, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, CyTOF-ready
• Gene Alias: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGFR variant III, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone DH8.3 of EGF R/ErbB1 antibody is specific for Truncated EGFR which is also called EGFR type III, EGFRvIII or delta-EGFR (characterized by a deletion of 267 amino acids in the extracellular domain, leading to a receptor which is unable to bind ligand yet is constitutively active). Because of its impaired internalisation and degradation, EGFRvIII is known to enhance tumourigenesis. NOTE: Clone DH8.3 DOES NOT cross-react with full length or wildtype EGFR (wtEGFR).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, CyTOF
• Clone: DH8.3
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% Sodium Azide
• Gene Symbols: EGFR
• Immunogen: Synthetic peptide corresponding to the junctional region of the truncated receptor LEEKKGNYVVTDHC,conjugated to keyhole limpet haemcyanin.
• Quantity: 0.1 mL
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Cellular Signaling, Growth and Development, Hypoxia, Phospho Specific, Signal Transduction, Tumor Suppressors, Tyrosine Kinases
• Gene ID (Entrez): 1956
• Target Species: Human
• Form: Purified