Invitrogen™ EGFRvIII Recombinant Rabbit Monoclonal Antibody (RM419)

Catalog No.	Quantity
PIMA536216	100 μL

Catalog No. PIMA536216 Supplier Invitrogen™ Supplier No. MA536216

Description

Rabbit Recombinant Monoclonal Antibody

MA5-36216 reacts to human Epidermal Growth Factor Receptor Variant III. No cross reactivity with wild type EGF receptor.

EGFRvIII is included in receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

Specifications

• Antigen: EGFRvIII
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: RM419
• Concentration: 0.02 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide
• Gene: Egfr
• Gene Accession No.: P00533
• Gene Alias: 6720426O10Rik; 9030024J15Rik; A830025P08Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; Cntn5; CNTN6; contactin 5; contactin 6; contactin-5; Contactin-6; EGFR; EGFR-related peptide; epidermal growth factor receptor; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); Erbb; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; Gm507; HER1; hNB-2; HNB-2s; hNB-3; mENA; mNB-3; Nb-2; NB3; NB-3; neural adhesion molecule; neural recognition molecule NB-2; Neural recognition molecule NB-3; NISBD2; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; wa2; wa-2; Wa5; waved 2
• Gene Symbols: Egfr
• Host Species: Rabbit
• Immunogen: A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII) (aa 1-100).
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1956
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG