Invitrogen™ eIF3f Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA596549

Catalog No. PIPA596549

Description

Immunogen sequence: GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL; Positive Samples: HL-60, SKOV3, BT-474, Jurkat, HeLa, Mouse spleen, Mouse lung, Mouse heart, Rat brain; Cellular Location: Cytoplasm.

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Specifications

• Antigen: eIF3f
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EIF3F
• Gene Alias: 0610037M02Rik; AA409853; deubiquitinating enzyme eIF3f; eIF3 p47; eIF-3-epsilon; eIF3-epsilon; EIF3F; eIF3-p47; Eif3s5; eukaryotic translation initiation factor 3 subunit 5; eukaryotic translation initiation factor 3 subunit 5 epsilon; eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3, subunit 5 (epsilon); eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; eukaryotic translation initiation factor 3, subunit F
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 293427, 66085, 8665
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 4.31 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O00303, Q9DCH4
• Gene Symbols: EIF3F
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 88-357 of human EIF3F (NP_0037451).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov