Invitrogen™ eIF3l Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA531647

Catalog No. PIPA531647

Description

Recommended positive controls: mouse brown, white adipose. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (94%), Dog (100%), Pig (100%), Chicken (98%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). [UniProt].

Specifications

• Antigen: eIF3l
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EIF3L
• Gene Alias: 0610011H21Rik; 66 kDa tyrosine-rich heat shock protein; 67 kDa polymerase-associated factor; D15N1e; eIEF associated protein HSPC021; EIF3EIP; Eif3ip; EIF3L; EIF3S11; Eif3s6ip; Eukaryotic translation initiation factor 3 subunit 6-interacting protein; eukaryotic translation initiation factor 3 subunit E-interacting protein; Eukaryotic translation initiation factor 3 subunit L; eukaryotic translation initiation factor 3, subunit 6 interacting protein; eukaryotic translation initiation factor 3, subunit L; HSP-66Y; HSPC021; HSPC025; MSTP005; Paf67
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 223691, 51386
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q8QZY1, Q9Y262
• Gene Symbols: EIF3L
• Immunogen: Recombinant fragment corresponding to a region within amino acids 330 and 564 of Human EIF3L.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG