Invitrogen™ eIF4E Monoclonal Antibody (5D11)

Catalog No.	Quantity
PIMA1089	100 μg

Catalog No. PIMA1089 Supplier Invitrogen™ Supplier No. MA1089

Description

Mouse Monoclonal Antibody

MA1-089 detects eIF4E in human and mouse samples and has been successfully used in Western blot, immunofluorescence, immunocytochemistry and immunoprecipitation applications. MA1-089 does not react with rat eIF4E. The MA1-089 immunogen is full length human eIF4E produced in HEK293T cells.

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Specifications

• Antigen: eIF4E
• Applications: Immunoprecipitation, Western Blot, RNA Immunoprecipitation, Immunocytochemistry
• Classification: Monoclonal
• Clone: 5D11
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene: Eif4e
• Gene Accession No.: P06730, P63073
• Gene Alias: AUTS19; CBP; EG668879; EIF4E; EIF-4E; EIF4E1; EIF4EL1; Eif4e-ps; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; eukaryotic translation initiation factor 4E-like protein; eukaryotic translation initiation factor 4-like protein; If4e; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705; translation initiation factor eIF-4E
• Gene Symbols: Eif4e
• Host Species: Mouse
• Immunogen: Full length human eIF4E produced in HEK293T cells.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13684, 1977
• Target Species: Human, Mouse
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1