Invitrogen™ eIF4E Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA577960

Catalog No. PIPA577960

Description

Positive Control: 293T, 293T cytoplasm fraction extract, mouse colon Predicted Reactivity: Rat (99%), Xenopus laevis (91%), Rabbit (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Specifications

• Antigen: eIF4E
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Eif4e
• Gene Alias: AUTS19; CBP; EG668879; EIF4E; EIF-4E; EIF4E1; EIF4EL1; Eif4e-ps; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; eukaryotic translation initiation factor 4E-like protein; eukaryotic translation initiation factor 4-like protein; If4e; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705; translation initiation factor eIF-4E
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13684, 1977
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.52 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P06730, P63073
• Gene Symbols: Eif4e
• Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG