MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLacI Competent Cells

Catalog No.	Quantity
71-353-3	0.4 mL
71-353-4	1 mL

Catalog No. 71-353-3 Supplier MilliporeSigma™ Supplier No. 713533

Description

Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.

Expression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA. Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors. Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs. In Rosetta-gami 2(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Specifications

• Efficiency: Guaranteed efficiency: >2 x 10⁶ cfu/μg
• Content And Storage: ≤-70°C
• Quantity: 0.4 mL