MilliporeSigma™ ReBlot Plus Kit and Reagents

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

Supplier:  MilliporeSigma™ 2502

Catalog No. 2502MI

Includes: Mild Antibody Stripping Solution (10X), 50mL; Strong Antibody Stripping Solution (10X), 50mL

Description

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electrophoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid-based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.

• No pungent-smelling β-mercaptoethanol is contained in the Antibody Stripping Solution
• Antibody stripping is done at room temperature—no heating of blots is required
• Blots may be reused in 25 min.

Specifications

• Product Type: Mild WB Stripping Buffer
• Quantity: 50 mL
• Kit Contents: 25mL Mild Antibody stripping solution 10X

• For Use With (Application): Western Blot
• Concentration: 2X
• Content And Storage: 2°C to 8°C