MilliporeSigma™ ReBlot Plus Kit and Reagents
Blots can be stripped of antibodies in approximately 15 minutes at room temperature
Supplier: MilliporeSigma™ 2502
Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electrophoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid-based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.
- No pungent-smelling β-mercaptoethanol is contained in the Antibody Stripping Solution
- Antibody stripping is done at room temperature—no heating of blots is required
- Blots may be reused in 25 min.
|Western Blot Stripping Buffer|
|25mL Mild Antibody stripping solution 10X|
|2°C to 8°C|
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