Invitrogen™ eNOS Monoclonal Antibody (M221)

Catalog No.	Quantity
PIMA547674	100 μL

Catalog No. PIMA547674 Supplier Invitrogen™ Supplier No. MA547674

Description

Mouse Monoclonal Antibody

The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells.

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

Specifications

• Antigen: eNOS
• Applications: Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: M221
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: NOS3
• Gene Accession No.: P29474, P70313, Q62600
• Gene Alias: 2310065A03Rik; cNOS; Constitutive NOS; Ecnos; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial nitric oxide synthase NOS3; endothelial NOS; eNos; e-NOS; nitric oxide synthase; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3, endothelial cell; nitric oxide synthase, endothelial; NOS; NOS type III; Nos3; Nos-3; NOSIII; OTTHUMP00000213183
• Gene Symbols: NOS3
• Host Species: Mouse
• Immunogen: Clone (M221) was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18127, 24600, 4846
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1