Invitrogen™ eNOS Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1037

Catalog No. PA1037

Description

PA1-037 detects endothelial nitric oxide synthase (eNOS) from bovine, canine, human, mouse, porcine and rat tissues as well as recombinant protein. This antibody does not detect human iNOS or rat nNOS. PA1-037 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. By Western blot PA1-037 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in human vascular endothelial cells with PA1-037 results in prominent staining of the epithelial cells. The PA1-037 immunogen is a synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

Specifications

• Antigen: eNOS
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOS3
• Gene Alias: 2310065A03Rik; cNOS; Constitutive NOS; Ecnos; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial nitric oxide synthase NOS3; endothelial NOS; eNos; e-NOS; nitric oxide synthase; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3, endothelial cell; nitric oxide synthase, endothelial; NOS; NOS type III; Nos3; Nos-3; NOSIII; OTTHUMP00000213183
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18127, 24600, 287024, 397557, 403784, 4846
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P29473, P29474, P70313, Q28969, Q62600, Q9TUX8
• Gene Symbols: NOS3
• Immunogen: Synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Bovine, Pig
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov