Invitrogen™ EOMES Monoclonal Antibody (Dan11mag), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 14487580

Catalog No. 50-245-555

Description

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. Applications Reported: This Dan11mag antibody has been reported for use in immunohistochemistry of formalin-fixed paraffin embedded tissue sections, intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated Dan11mag is recommended for use in intracellular flow cytometry.). Applications Tested: This Dan11mag antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes and/or western blotting at 5 μg/mL. This Dan11mag antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low pH antigen retrieval at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

Specifications

• Antigen: EOMES
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O54839
• Gene Symbols: Eomes
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13813
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: Dan11mag
• Conjugate: Unconjugated
• Gene: Eomes
• Gene Alias: C77258; EOMES; eomesodermin; eomesodermin homolog; eomesodermin homolog (Xenopus laevis); T-box brain protein 2; T-box transcription factor; TBR2; TBR-2; T-brain-2
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.