MilliporeSigma™ Eosinophil Cationic Protein, Mouse, Unlabeled, Clone: 1C8.5/D8,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF986

Catalog No. MABF986MI

Description

Eosinophil cationic protein (UniProt P12724; also known as ECP, Ribonuclease 3, RNase 3) is encoded by the RNASE3 (also known as ECP, RNS3) gene (Gene ID 6037) in human. The major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO), are eosinophil effector molecules implicated in host defense, immunoregulatory responses, and in allergic and inflammatory reactions. These highly cationic proteins are stored preformed within the eosinophil secondary granules and released in intact membrane-bound granules during cell lysis or through a complex system of vesiculotubular structures during “piecemeal necrosis”. While eosinophil count and eosinophil surface expression of the activation marker CD69 are significantly correlated with serum concentrations of MBP, EDN and EPO, studies show that they are not correlated with ECP level. On the other hand, eosinophilic disease patients with normal eosinophil count show significantly increased concentrations of MBP, ECP, EDN, and EPO compared to normal donors. The measurement of MBP, ECP, EDN, and EPO levels thus provides a better predictor than eosinophil count alone in disease diagnosis. ECP is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature (a.a. 28-160) protein.

Specifications

• Antigen: Eosinophil Cationic Protein
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG1 antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: P12724
• Immunogen: Purified human ECP.
• Quantity: 100 μg
• Research Discipline: Inflammation & Immunology
• Gene ID (Entrez): NP_002926
• Target Species: Human
• Form: Purified

• Applications: Immunohistochemistry (Paraffin), Immunoassay, Western Blot
• Clone: 1C8.5/D8
• Conjugate: Unconjugated
• Gene: RNASE3, ECP, RNS3
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 1C8.5/D9 specifically reacted with ECP, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG1

For Research Use Only. Not for use in diagnostic procedures.