Invitrogen™ EPCR Monoclonal Antibody (RCR-252)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA533375

Catalog No. PIMA533375

Description

Product is supplied at 1 mL (100 μL/mL). Application Notes: Flow cytometry- This antibody stains the extracellular domain of human EPCR. RE-1 cells were used as a positive control and N1 cells were used as a negative control. Western blot - A non-reduced sample treatment was used. The observed band size is 50 kDa. Functional Assay: This antiboyd functions as blocking antbody. It ablates APC binding to EPCR and blocks APC-mediated signaling. The antibody was functionally tested by pretreatment of cells with 10-25 μg/mL RCR-252. Positive control: RE-1 (rat 3Y1 fibroblasts stably transfected with human EPCR); Negative control: N1 cells.

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

Specifications

• Antigen: EPCR
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.1% BSA and no preservative
• Gene Accession No.: Q9UNN8
• Gene Symbols: Procr
• Immunogen: Human EPCR-positive RE-1 cells.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Clone: RCR-252
• Conjugate: Unconjugated
• Gene: Procr
• Gene Alias: Activated protein C receptor; AI325044; APC receptor; bA42O4.2; CCCA; CCD41; CD antigen CD201; CD201; CD201 antigen; cell cycle, centrosome-associated protein; centrocyclin; Centrosomal protein CCD41; endothelial cell protein C receptor; Endothelial protein C receptor; Epcr; PROCR; protein C receptor; protein C receptor, endothelial
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 10544
• Content And Storage: 4°C
• Form: Liquid