EphA2, Human anti-Human, Clone: 1C1, Novus Biologicals™

Human Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB015313	Novus Biologicals NBP2810690.025MG	0.025 mg
NB015314	Novus Biologicals NBP2810690.2MG	0.2 mg

Description

EphA2 Monoclonal specifically detects EphA2 in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Block/Neutralize.

Specifications

• Antigen: EphA2
• Concentration: 1 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunofluorescence, Blocking Assay
• Conjugate: Unconjugated
• Host Species: Human
• Research Discipline: Cancer, Protein Kinase
• Formulation: PBS
• Gene ID (Entrez): 1969
• Immunogen: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Clone: 1C1
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Block/Neutralize
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: ARCC2, EC 2.7.10, EC 2.7.10.1, ECKepithelial cell receptor protein tyrosine kinase, EPH receptor A2, EphA2, ephrin type-A receptor 2, Epithelial cell kinase, soluble EPHA2 variant 1, Tyrosine-protein kinase receptor ECK
• Gene Symbols: EPHA2
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ∽0.8 nM; Fab KD ∽140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.