Invitrogen™ EphA2 Recombinant Human Monoclonal Antibody (1C1)

Human Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA547953

Catalog No. PIMA547953

Description

Specificity: 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.

EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Specifications

• Antigen: EphA2
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: P29317
• Gene Symbols: EPHA2
• Immunogen: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry
• Clone: 1C1
• Conjugate: Unconjugated
• Gene: EPHA2
• Gene Alias: ARCC2; AW545284; CTPA; CTPP1; CTRCT6; Eck; EPH receptor A2; Epha2; ephrin receptor EphA2; Ephrin type-A receptor 2; Epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; I79_019726; Myk2; Sek2; Sek-2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; tyrosine-protein kinase receptor SEK-2
• Host Species: Human
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 1969
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid