EphB2 Mouse anti-Human, Brilliant Violet 650, Clone: 2H9, BD Optibuild™

Catalog No.	Quantity
BDB743765	50 μg

Catalog No. BDB743765 Supplier BD Biosciences Supplier No. 743765

Description

Mouse Monoclonal Antibody

The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2). EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors. EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B). Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems. The EphB2 receptor functions as a chemodirectant in cellular migration. EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, influencing cell fate, morphogenesis and organogenesis. In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions. Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation. Ephrin-B2 molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes. This interaction promotes monocyte differentiation into proinflammatory macrophages. In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression. These include intestinal stem cells (ISCs) which express high levels of EphB2. The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

Specifications

• Antigen: EphB2
• Classification: Monoclonal
• Clone: 2H9
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 650
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: EPHB2, EphB2R, TYRO5, CAPB, DRT, EK5, EPHT3, EPTH3, HEK5, PCBC
• Host Species: Mouse
• Immunogen: Human EphB2 Recombinant Protein
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov