Invitrogen™ Human EPO ProQuantum Immunoassay Kit

Description

ELISA

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.

Specifications

• Accession Number: P01588
• Assay Range: 1.28 to 20000 mIU/mL
• Assay Sensitivity: <1 mlU/mL
• Conjugate: FAM™
• Detection Method: Taqman™ Real-time PCR
• Immunoassay Kit Format: Proximity-based Immunoassay
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Protein Quantitation
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 2056
• Incubation Time: 1 hr.
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Label or Dye: FAM™
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: Frozen at -20°C
• Target: Epo
• Test Time: 30 mins
• Total Assay Time: 2 hrs

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov