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PeproTech Epo Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P318100
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500P318100 100 μg
500P31850UG 50 μg
500P3181MG 1 mg
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Catalog No. 500P318100 Supplier PeproTech Supplier No. 500P318100UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography employing an immobilized Human EPO matrix. Sandwich ELISA: To detect Human EPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human EPO (500-P318Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human EPO. Western Blot: To detect Human EPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Erythropoietin (EPO) protein is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. EPO is not responsible for early commitment to the lineage, and instead induces the final maturation of partially differentiated erythroid cells and the production of red cell-specific proteins, such as hemoglobin. The EPO receptor (EPOR) is present in higher levels on precursor cells, and expression diminishes with cell maturation.Hepatocytes are the main source of EPO in the fetus, followed by interstitial cells of the kidney after birth and through adulthood. EPO expression is upregulated during hypoxia, and low levels of transcription have also been detected in lung and brain tissue under these conditions. Recent data suggest that EPO may also play a role in the protection of neuronal tissue from damage and initiation of repair.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Epo
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene EPO
Gene Accession No. P01588
Gene Alias EP; EPO; Epoetin; erythropoietin; MGC138142; MVCD2
Gene Symbols EPO
Host Species Rabbit
Immunogen CHO cells-derived Recombinant Human EPO.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 2056
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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