Invitrogen™ Epstein Barr Virus Nuclear Antigen 1 Monoclonal Antibody (0212)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547602

Catalog No. PIMA547602

Description

Specificity: specific for EBNA-1 protein. Reactive with native antigen. Epitope lies between aa 408-641. Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography. The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Each vial contains 1.0 mL. This product contains no stabilizing proteins and should be stored at 4C until ready for use. Working dilution must be determined by the user. Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200. for ELISA.

Epstein-Barr virus (EBV) is a human herpes virus, which is associated with conditions such as Hodgkin's disease and Burkitt's Lymphoma and is the causative agent in mononucleosis in adolescents. EBV latently infects B lymphocytes. Infected B cells express EBV nuclear antigens and latent proteins LMP1, LMP2A and LMP2B. LMP2A forms aggregates in the plasma membranes of B lymphocytes, where it functions as a negative regulator of the Src and Syk protein tyrosine kinases. Studies show that LMP2A blocks B-cell receptor (BCR) signal transduction in EBV immortalized B cells in vitro and may play an important role in maintaining a latent EBV infection within the peripheral blood B cells of infected individuals.EBNA-1 is the only viral protein consistently expressed in all malignancies associated with EBV.

Specifications

• Antigen: Epstein Barr Virus Nuclear Antigen 1
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.2
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Virus
• Product Type: Antibody
• Isotype: IgG2a

• Applications: ELISA, Western Blot
• Clone: 0212
• Conjugate: Unconjugated
• Gene Alias: EBNA-1
• Immunogen: Proprietary sequence.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov