ErbB4, Rabbit anti-Mouse, Clone: mab10, Millipore Sigma™

Catalog No.	Quantity
MABN248425	25 μL
MABN2484100	100 μL

Catalog No. MABN248425 Supplier MilliporeSigma Supplier No. MABN248425UL

Description

Rabbit Monoclonal Antibody

Anti-ErbB4, clone mAB10, Cat. No. MABN2484, is a highly specific rabbit monoclonal antibody that targets Receptor tyrosine-protein kinase ErbB-4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Receptor tyrosine-protein kinase erbB-4 (UniProt: Q61527; also known as EC: 2.7.10.1, Proto-oncogene-like protein c-ErbB-4) is encoded by the Erbb4 (also known as Mer4) gene (Gene ID: 13869) in murine species. ErbB-4 is a single-pass type I membrane protein of the EGF receptor family that plays an essential role as cell surface receptor for neuregulins (NRG) and EGF family members. ErbB-4 is monomeric in the absence of bound ligand and it homo- or heterodimerizes with another ERBB family member upon ligand binding. ErbB-4 is autophosphorylated on tyrosine residues in response to ligand binding that triggers receptor dimerization and autophosphorylation at specific tyrosine residues, which then serve as binding sites for scaffold proteins and effectors. ErbB-4 is reported to regulate the development of the heart, the central nervous system, and the mammary gland. It is shown to be involved in gene transcription, cell proliferation, differentiation, migration, and apoptosis. It is expressed in the developing and adult cerebral cortex, and represents the major NRG receptor in central neurons and regulates a diverse array of neuronal processes, including migration, differentiation, neurotransmission, and synaptic plasticity. NRG-1 and ErbB-4 are essentials of a signaling pathway that is critical for the proper development of inhibitory cortical and hippocampal circuits. ErbB-4 expression is reported to be restricted to GABAergic neurons in the cerebral cortex where it plays a role in regulating GABAergic synaptic development and interneuron migration. ErbB-4 is synthesized with a signal peptide (aa 1-25), which is cleaved off in the mature form. The mature form has an extracellular domain (aa 26-652), a short transmembrane domain (aa 653-673), and a long cytoplasmic tail (aa 674-1308). Its protein kinase domain is localized in the cytoplasmic tail (aa 718-985). Three isoforms of ErbB-4 have been described that are produced by alternative splicing.

Specifications

• Antigen: ErbB4
• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: mab10
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected ErbB4 in mouse embryo tissue sections.Western Blotting Analysis: A representative lot detected ErbB4 in Western Blotting applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).ELISA Analysis: A representative lot detected ErbB4 in ELISA applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).Immunohistochemistry Analysis: A representative lot detected ErbB4 in Immunohistochemistry applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).Immunoprecipitation Analysis: A representative lot immunoprecipitated ErbB4 in Immunoprecipitation applications (Vullhorst, D., et. al. (2009). J Neurosci. 29(39):12255-64).
• Formulation: Purified rabbit monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Receptor tyrosine-protein kinase erbB-4;Proto-oncogene-like protein c-ErbB-4
• Gene Symbols: Erbb4;Mer4
• Host Species: Rabbit
• Immunogen: GST-tagged recombinant protein corresponding to 204 amino acids from the C-terminal region of murine Receptor tyrosine-protein kinase erbB-4.
• Purification Method: Protein G purified
• Quantity: 25 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_034284.1
• Test Specificity: Clone mAB10 is a rabbit monoclonal antibody that detects mouse Receptor tyrosine-protein kinase erbB-4. It targets an epitope with in the C-terminal region.
• Target Species: Mouse
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG