Invitrogen™ ERH Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521388

Catalog No. PIPA521388

Description

Recommended positive controls: A549, H1299, HCT116, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (100%), Pig (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The Enhancer of Rudimentary Homolog (ERH) is a highly conserved protein with significant roles in the regulation of gene expression and cell cycle control. Structurally, ERH forms homodimers, characterized by a crystal structure resolving at 2.0 A, which reveals its ability to bind specific protein regions via a hydrophobic groove. Functionally, ERH interacts with various cellular components, notably integrating into the Microprocessor complex where it facilitates microRNA maturation by binding with the N-terminus of DGCR8 at a 2:2 stoichiometry. This interaction underscores ERH's role in gene silencing, contributing to the biogenesis of small interfering RNAs (siRNAs). Additionally, ERH is implicated in transcriptional regulation and has been associated with DNA replication, suggesting its involvement in broader genomic functions. Despite its diverse roles, ERH operates in a plug-and-play manner, allowing its interaction with different cellular factors to fulfill pleiotropic functions across metazoans.

Specifications

• Antigen: ERH
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ERH
• Gene Alias: DROER; Enhancer of rudimentary homolog; enhancer of rudimentary homolog (Drosophila); Erh; Mer; Prei1; preimplantation protein 1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13877, 2079
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.79 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P84089, P84090
• Gene Symbols: ERH
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ERH. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG