Invitrogen™ ERK1 Polyclonal Antibody

Catalog No.	Quantity
PIPA594966	100 μg

Catalog No. PIPA594966 Supplier Invitrogen™ Supplier No. PA594966

Description

Rabbit Polyclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: Rat Spleen Tissue, Rat Thymus Tissue, Rat Skeletal Muscle Tissue, Rat Kidney Tissue, HELA Cell, JURKAT Cell, RAJI Cell, COLO320 Cell. IHC: Human Mammary Cancer Tissue, rat intestine tissue. ICC/IF: HELA Cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.

Specifications

• Antigen: ERK1
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene: MAPK3
• Gene Accession No.: P21708, P27361, Q63844
• Gene Alias: ERK; Erk1; ERK-1; Ert2; Esrk1; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAP kinase2; MAPK 1; MAPK 3; MAPK1; MAPK2; MAPK3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 3; mitogen-activated 3; mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; MNK1; Mtap2k; p44; p44 MAP kinase; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; pp42/MAP kinase; PRKM3
• Gene Symbols: MAPK3
• Host Species: Rabbit
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ERK1 (287-301aa KTKVAWAKLFPKSDS).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 26417, 50689, 5595
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG