Invitrogen™ ERp57 Monoclonal Antibody (4F9), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545375

Catalog No. PIMA545375

Description

A 1:1,000 dilution of MA5-45375 was sufficient for detection of ERp57 in 15 μg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Detects approximately 57 kDa protein.

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Specifications

• Antigen: ERp57
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4
• Gene Accession No.: P30101
• Gene Symbols: PDIA3
• Immunogen: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot, Immunocytochemistry
• Clone: 4F9
• Conjugate: FITC
• Gene: PDIA3
• Gene Alias: 58 kDa glucose-regulated protein; 58 kDa microsomal protein; 58kDa; Disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum protein; endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER p57; ER protein 57; ER protein 60; ER60; ER-60 protease; Erp; ERp 57; ERp57; ERP57 protein; ERP60; ERp61; glucose regulated protein 58; glucose regulated protein 58kD; glucose regulated protein, 58 kDa; glucose regulated protein, 58kDa; GRP 57; GRP 58; GRP57; Grp58; grp-58; HEL-S-269; HEL-S-93n; HIP-70; HsT17083; I79_003765; oxidoreductase ERp57; p58; PDI; PDIA 3; PDIA3; PDI-Q2; phospholipase C, alpha; phospholipase C-alpha; PI PLC; PI-PLC; PLC alpha; PLC[a]; Plca; protein disulfide isomerase associated 3; protein disulfide isomerase family A member 3; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; protein disulfide-isomerase A3; Q-2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 2923
• Content And Storage: 4°C, store in dark
• Form: Liquid

Safety and Handling

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