Invitrogen™ ESAM Monoclonal Antibody (1G8), PE, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-109-05	Invitrogen™ 12585282	100 μg

Description

Description: The 1G8 monoclonal antibody reacts with mouse ESAM (endothelial cell-selective adhesion molecule). ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils. Applications Reported: This 1G8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1G8 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

Specifications

• Antigen: ESAM
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q925F2
• Gene ID (Entrez): 69524
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography

• Clone: 1G8
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Mouse
• Gene: ESAM
• Gene Alias: 2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; endothelial cell-specific adhesion molecule; Esam; Esam1; HUEL (C4orf1)-interacting protein; LP4791 protein; UNQ220/PRO246; W117m
• Gene Symbols: ESAM
• Product Type: Antibody