Esrp-1/2 Monoclonal Antibody (23A7.C9), Rockland™

Catalog No.	Quantity
50-199-1459	100 μg
50-199-1460	25 μL

Catalog No. 50-199-1459 Supplier Rockland Immunochemicals Supplier No. 210301C31

Description

Mouse Monoclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing program induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.

Specifications

• Antigen: Esrp-1/2
• Applications: ELISA, Western Blot
• Classification: Monoclonal
• Clone: 23A7.C9
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: ESRP2
• Gene Accession No.: Q8K0G8
• Gene Alias: 9530027K23Rik; Epithelial splicing regulatory protein 2; Esrp2; PP7059; Rbm35b; RNA binding motif protein 35A; RNA binding motif protein 35b; RNA-binding motif protein 35B; RNA-binding protein 35B
• Gene Symbols: ESRP2
• Host Species: Mouse
• Immunogen: Anti-Esrp-1/2 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 77411
• Target Species: Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ