Invitrogen™ Estriol ELISA Kit

Description

ELISA

The Estriol Competitive ELISA quantitates estriol in serum, plasma, urine, saliva, dried fecal extracts or or cell culture medium.∣Principle of the method: The Estriol Competitive ELISA research-use-only kit is designed to quantitatively measure estriol independent of species. An estriol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit estriol antibodies and estriol-peroxidase conjugate is added to the wells. The estriol-peroxidase conjugate and any estriol in the sample will compete to bind to the anti-rabbit estriol antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound estriol-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of estriol present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Estriol is the main estrogen in the blood and urine of pregnant females. Serum estriol determinations reflect the status of the fetus and are used to evaluate fetal distress and placental function.

Specifications

• Accession Number: C18H24O3; Estratriol; Estriol; Oestriol; Ovestin; Ovestrion
• Assay Range: 19.66 to 12,000 pg/mL
• Assay Sensitivity: 5.33 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 12%
• Intraassay CV: 10
• Kit Contents: Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
• Storage Requirements: -20°C
• Target: Estriol
• Test Time: 45 mins
• Total Assay Time: 2 hrs 30 mins