ETF1 Mouse anti-Human, Clone:4F9H12, Abnova™

Mouse monoclonal antibody raised against recombinant human ETF1.

Supplier:  Abnova Corporation MAB17190

Catalog No. 89-333-571

Description

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Specifications

• Antigen: ETF1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: ELISA (1:10000)
  Western Blot (1:500-1:2000)
  Immunohistochemistry
  Immunocytochemistry
  Flow Cytometry (1:200-1:400)
  The optimal working dilution should be determined by the end user.
• Gene: ETF1
• Host Species: Mouse
• Quantity: 100 μg
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Western Blot
• Clone: 4F9H12
• Description: eukaryotic translation termination factor 1
• Formulation: In PBS (0.05% sodium azide)
• Gene Alias: D5S1995, ERF, ERF1, MGC111066, RF1, SUP45L1, TB3-1
• Immunogen: Recombinant protein corresponding to amino acid 288-437 of human ETF1 from E. coli.
• Gene ID (Entrez): 2107
• Content And Storage: Store at 4°C. For long term storage store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid