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Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, Universal Non-distribution product as customer accommodation.

Incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments.

Manufacturer:  Invitrogen™ 11781200

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Catalog No. 11-781-200


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Description

Description

  • Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase - complete, rapid activation provides the best sensitivity and reproducibility
  • The One-Step SuperScript™ III Reverse Transcriptase module - a unique formulation to ensure better yield and less PCR inhibition, specifically for one-step qRT-PCR
  • Flexible formats with ROX premixed or separate - designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler™ 480, Corbett Rotor-Gene™)
  • The only commercially available one-step qRT-PCR kits containing heat-labile UDG - significantly reduced carryover contamination risk with excellent activity from room temperature to approximately 40°C
  • Due to the thermostability of SuperScript™ III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated

Easy to test and switch:
  • EXPRESS kits work with manufacturer's default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience
  • The kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs
Specifications

Specifications

Primer-Probe Detection
7500 Fast System, 7500 System, BioRad CFX96™, BioRad MyiQ, BioRad iCycler iQ, Cepheid SmartCycler, Corbett Rotor-Gene, MJ Chromo4, MJ Opticon, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
RNA
Store at -20°C for long-term storage. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4° to 8°C.
Real time PCR (qPCR), real time PCR-based gene expression profiling
The EXPRESS One-Step SuperScript™ qRT-PCR Universal kit contains 3 modules: 2X EXPRESS SuperScript™ Mix (1mL), Express qPCR SuperMix Universal (5mL), and ROX Reference Dye (500μL).
Fast, Standard
500 Reactions
Documents
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