Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format

Description

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Cleavable - disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
• Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length - spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain

Specifications

• Product Type: Sulfo-NHS-SS-Biotin
• Quantity: 10 x 1 mg
• Content And Storage: Store at -20°C.
• Labeling Method: Chemical Labeling
• Shipping Condition: Wet Ice
• Product Line: EZ-Link™
• Cell Permeability: Cell-Impermeant
• Chemical Reactivity: Amine
• Reactive Moiety: Sulfo-NHS Ester
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
• Label or Dye: Biotin
• Spacer: Cleavable, 24.3 Å

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.