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F(Ab')2 Fluorescein Goat anti-Chemical, AP, Polyclonal, Rockland™
SDP

Catalog No. 501992596
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50-199-2596 1 mg
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Catalog No. 50-199-2596 Supplier Rockland Immunochemicals Supplier No. 700105096
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Description

Goat Polyclonal Antibody

Store vial at 4°C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

Specifications

Antigen F(Ab')2 Fluorescein
Applications ELISA, Immunohistochemistry, Western Blot
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Alkaline Phosphatase (AP)
Formulation 0.05M tris HCl with 0.0001M ZnCl2, 0.001M MgCl2, 0.15M NaCl, 10 mg/mL BSA, 50% glycerol and 0.1% sodium azide; pH 8
Gene Alias Fluorescein
Host Species Goat
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Primary
Content And Storage 4°C, do not freeze
Product Type Antibody
Form Liquid
Target Molecule Chemical
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