DescriptionThe Recombinant F. meningosepticum PNGase F Protein is derived from E. coli. The Recombinant F. meningosepticum PNGase F Protein has been validated for the following applications: Enzyme Activity.
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|Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.|
|E. coli-derived Ala41-Asn354 with N-terminal Met and 6-His tag|
|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining|
|Observed M.W.: 34kDa, reducing conditions, Theoretical M.W.: 36kDa|
|<1.0 EU per 1μg of the protein by the LAL method.|
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