Invitrogen™ F4/80 Monoclonal Antibody (Cl:A3-1), FITC

Catalog No.	Quantity
PIMA516628	50 μg
PIMA516627	100 μg

Catalog No. PIMA516628 Supplier Invitrogen™ Supplier No. MA516628

Description

Rat Monoclonal Antibody

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a approximately 160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Specifications

• Antigen: F4/80
• Applications: Flow Cytometry, Immunocytochemistry
• Classification: Monoclonal
• Clone: Cl:A3-1
• Concentration: 0.1 mg/mL
• Conjugate: FITC
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Gene: ADGRE1
• Gene Accession No.: Q61549
• Gene Alias: ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
• Gene Symbols: ADGRE1
• Host Species: Rat
• Immunogen: Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
• Purification Method: Protein G
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13733
• Target Species: Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b