Invitrogen™ F4/80 Monoclonal Antibody (SP115)

Catalog No.	Quantity
PIMA516363	500 μL

Catalog No. PIMA516363 Supplier Invitrogen™ Supplier No. MA516363

Description

Rabbit Monoclonal Antibody

This antibody is predicted to react with rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. A suggested positive control is human lung or mouse liver tissue.

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Specifications

• Antigen: F4/80
• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: SP115
• Concentration: 0.05 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.2
• Gene: ADGRE1
• Gene Accession No.: Q61549
• Gene Alias: ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
• Gene Symbols: ADGRE1
• Host Species: Rabbit
• Immunogen: Synthetic peptide derived from N-terminus of mouse F4/80 protein.
• Purification Method: Protein A
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13733
• Target Species: Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG