Invitrogen™ FAM105B Polyclonal Antibody

Catalog No.	Quantity
PIPA5143981	100 μg

Catalog No. PIPA5143981 Supplier Invitrogen™ Supplier No. PA5143981

Description

Rabbit Polyclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human HELA whole cell, human THP-1 whole cell, human K562 whole cell, rat liver tissue, mouse liver tissue. IHC: human appendicitis tissue, human bladder cancer tissue, human tonsil tissue, human placenta tissue. ICC/IF: SIHA cell. Flow: 293T cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling.

Specifications

• Antigen: FAM105B
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.01mg sodium azide
• Gene: OTULIN
• Gene Accession No.: Q3UCV8, Q96BN8
• Gene Alias: C79097; deubiquitinating enzyme otulin; FAM105B; family with sequence similarity 105, member B; GUM; gumby; m3Sapc; m7-1Sapc; OTU deubiquitinase with linear linkage specificity; OTU domain-containing deubiquitinase with linear linkage specificity; Otulin; protein FAM105B; ubiquitin thioesterase Gumby; Ubiquitin thioesterase otulin
• Gene Symbols: OTULIN
• Host Species: Rabbit
• Immunogen: E.coli-derived human OTULIN recombinant protein (Position: A49-L352).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100362554, 432940, 90268
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG