Invitrogen™ FAM21A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA571089

Catalog No. PIPA571089

Description

This target displays homology in the following species: Cow: 77%; Dog: 93%; Horse: 86%; Human: 100%; Pig: 85%; Rabbit: 79%; Rat: 77%.

Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes inhibits WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization and is involved in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission. Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35. Required for the endosomal recruitment of CCC complex subunits COMMD1 and CCDC93 as well as the retriever complex subunit VPS35L. [UniProt].

Specifications

• Antigen: FAM21A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WASHC2A
• Gene Alias: alternative protein FAM21B; bA56A21.1; bA98I6.1; FAM21A; FAM21B; family with sequence similarity 21 member A; family with sequence similarity 21, member A; family with sequence similarity 21, member B; WASH complex subunit 2A; WASH complex subunit FAM21A; WASH complex subunit FAM21B; WASHC2A
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 387680
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 2% sucrose and 0.09% sodium azide
• Gene Accession No.: Q641Q2
• Gene Symbols: WASHC2A
• Immunogen: synthetic peptide directed towards the C-terminal region of human FAM21A.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG